SPECIFICITY OF THE TEST BASED ON MODIFICATION OF CELL MORPHOLOGY FOR DETECTION OF LIPOPHILIC INHIBITORS OF PROTEIN PHOSPHATASES

Citation
Yf. Pouchus et al., SPECIFICITY OF THE TEST BASED ON MODIFICATION OF CELL MORPHOLOGY FOR DETECTION OF LIPOPHILIC INHIBITORS OF PROTEIN PHOSPHATASES, Toxicon, 35(7), 1997, pp. 1137-1142
Citations number
25
Categorie Soggetti
Toxicology,"Pharmacology & Pharmacy
Journal title
ISSN journal
00410101
Volume
35
Issue
7
Year of publication
1997
Pages
1137 - 1142
Database
ISI
SICI code
0041-0101(1997)35:7<1137:SOTTBO>2.0.ZU;2-A
Abstract
The test based on morphological changes in KB cells was assayed with d ifferent toxins. Only lipophilic inhibitors of protein phosphatases, s uch as okadaic acid or calyculin A, induced visible changes in cell mo rphology. The activity of contaminated mussel extracts on KB cells was evaluated comparatively by direct interpretation of morphological cha nges and by a colorimetric method estimating the number of viable cell s after staining. The latter technique revealed interferences (not det ected by the former) with mussel cytotoxins. These results show that t he technique, based on determination of the minimal active concentrati on of toxic extracts inducing morphological changes, is more specific, faster and preferable to the determination of IC50, for the detection of protein phosphatase inhibitors in shellfish. (C) 1997 Elsevier Sci ence Ltd.