J. Todorova et al., The effect of Cr(NO3)(3) on two model species of the family Chironomidae, Diptera - heat shock response and heat shock proteins 70, BIOLOGIA, 55(6), 2000, pp. 709-716
HSP 70 was analysed in two biologically different Chironomid species (Chiro
nomus riparius Mg. and Glyptotendipes pallens Mg.). Both species were chron
ically treated with three different concentrations of Cr(NO3)(3): 15.2 mug/
mL, 152 mug/mL, 304 mug/mL. Immunoblotting after separation of proteins by
gel electrophoresis showed that HSP 70 antibodies cross-react with the corr
esponding proteins of these two species. In both species the constitutive f
orm of HSP 70 was detected, which in G. pallens was much more pronounced. I
n larvae of both species treated with three different concentrations of Cr(
NO3)(3) additional amounts of HSP 70 appeared. They were slightly visible i
n C. riparius. In G. pallens with increasing the concentrations of Cr(NO3)(
3) more pronounced bands of these proteins were observed. Also, in G. palle
ns, treated with 304 mug/mL Cr(NO3)(3) an additional well visible band was
detected. HSP 70 was localised by indirect immunofluorescence in salivary g
land chromosomes AB and CD of G. pallens after chronic treatment with 304 m
ug/mL of Cr(NO3)(3). The different reactions of C. riparius and G. pallens
to stressful conditions induced by Cr(NO3)(3) are described in respect of t
heir biology. Having in mind a conservation of protective function, in view
of its importance, it could be expected that there would be a more effecti
ve protection mechanism in the mining species, G. pallens.