Enhancement of production of cloned alpha-amylase by lactic acid feeding from recombinant Saccharomyces cerevisiae using a SUC2 promoter

Authors
Zhang, XL Xia, ZJ Zhao, BX Cen, PL
Citation
Xl. Zhang et al., Enhancement of production of cloned alpha-amylase by lactic acid feeding from recombinant Saccharomyces cerevisiae using a SUC2 promoter, BIOTECH LET, 23(4), 2001, pp. 259-262
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
01415492 → ACNP
Volume
23
Issue
4
Year of publication
2001
Pages
259 - 262
Database
ISI
SICI code
0141-5492(200102)23:4<259:EOPOCA>2.0.ZU;2-S
Abstract
alpha -Amylase production was higher (13 units ml(-1)) when a recombinant S accharomyces cerevisiae containing a SUC2 promoter was grown with 10 g lact ic acid l(-1) than without addition (8 units ml(-1)). With continuous lacti c acid feeding in the inducing phase, alpha -amylase increased to 79 units ml(-1) in a 1-l jar fermenter.