Flt-1, vascular endothelial growth factor receptor 1, is a novel cell surface marker for the lineage of monocyte-macrophages in humans

Flt-1, also known as vascular endothelial growth factor receptor 1 (VEGFR-1 ), is a high-affinity tyrosine kinase receptor for VEGF and is expressed al most exclusively on vascular endothelial cells. As an exception, Flt-1 tran script was recently found to be expressed in human peripheral blood monocyt es, However, the protein of the Flt-1 receptor on the cell surface of monoc ytes is yet to be identified, and whether the Flt-1 protein is expressed du ring the differentiation of monocyte-macrophage lineage cells has not been examined. Using monoclonal antibodies against 2 different antigenic epitope s on the Flt-1 extracellular domain, this study found that the major popula tion of the monocyte-marker CD97(+) cells in human peripheral blood express Flt-1 as a cell surface molecule. VEGFR-2 (KDR/Flk-1) was not expressed at detectable levels in these cells. An Flt-1 neutralizing monoclonal antibod y significantly suppressed VEGF-induced migration of the monocytes, suggest ing an important role for Flt-1 in the biologic function of monocytes, Furt hermore, CD34(+) cells in human cord blood, originally negative for the Flt -1 expression, differentiated into Flt-1(+) cells in association with the a ppearance of monocyte-macrophage markers after a 5-week culture in the pres ence of hematopoietic cytokines, In addition, the Flt-1(+)CD11b(+) cell fra ction from CD34(+) cells was found to efficiently differentiate into multin uclear osteoclasts in the presence of macrophage colony-stimulating factor and osteoclast differentiation factor. These results strongly suggest that Flt-1 is a novel cell Surface marker as well as a biologically functional m olecule for monocyte-macrophage lineages in humans. (C) 2001 by The America n Society of Hematology.