Culture medium for amylase production by toxigenic fungi

Mycelial growth and amylase production by a mycotoxigenic strain of Fusariu m moniliforme and Aspergillus flavus were evaluated in a culture medium con taining starch, glycerol, wheat bran or cent. With emphasis on corn, differ ent fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. monilif orme growth. The medium composed of milky stage corn supernatant promoted t he best mycelial growth (p<0.05), and it was used to prepare amylase produc tion medium in the next step. The medium composed with 2% ground corn in mi lky stage corn supernatant (350g of milky stage corn blended with 250mL wat er and centrifuged) promoted the highest amylase production, which was at t he 10th day of fermentation, both for F. moniliforme (42.32U/mL) and A. fla vus (4,745.54U/mL).