Expression of facilitative glucose transporter 1 mRNA in colon cancer was not regulated by k-ras

The expression of facilitative glucose transporter isoforms in colon adenoc arcinoma and the possible role of k-ras in inducing GLUT (glucose transport er) mRNA were studied. RT-PCR demonstrated GLUT2 and GLUT3 expression in 10 0% of the ten normal colon mucosa samples but detected no GLUT1 mRNA. By co ntrast, GLUT1 mRNA was detected in all 20(100%) colon cancer samples examin ed. GLUT4 mRNA was not detected in either normal mucosa or colon cancer tis sues. Semiquantitative PCR demonstrated equal amounts of GLUT2 and GLUT3 mR NA in both normal mucosa and colon cancer samples. A point mutation in codo n 12 of k-ras was detected in only six of the 20 (30%) colon cancer samples . Thus, a major difference between normal colon epithelia and colon cancer was the acquisition of GLUT1 expression, which was unlikely to have been in duced by a point mutation in codon 12 Or k-ras. (C) 2000 published by Elsev ier Science Ireland Ltd. All rights reserved.