Urinary F-2-isoprostanes formation in kidney transplantation

Background: Oxygen free-radical mediated lipid peroxidation has been implic ated in many diseases such as chronic renal failure, hemodialysis and chron ic kidney transplant rejection. However, insight into the rule of free radi cal generation in kidney transplantation has been constrained by the limita tions of current indexes of oxidant stress in vivo. Isoprostaglandin F-2 al pha type-III (iPF(2 alpha)-III, formerly known as 8-iso-prostaglandin F-2 a lpha) is emerging as a reliable marker of oxidant stress in vivo. The purpo se of our study was to investigate iPF(2 alpha)-III formation as an index o f lipid peroxidation in the 5 d following kidney transplantation. Methods: Urinary iPF(2 alpha)-III measurements were performed by enzyme imm unoassay from day 1 to 5 in 11 patients undergoing kidney transplantation. Results were compared with 11 healthy volunteers matched in sex, age and ci garette smoking. Results: Urinary excretion of iPF(2 alpha)-III at day 1 did not significant ly differ between control and transplant group (111 +/- 17 vs. 92 +/- 10 pM /mM creatinine, respectively, NS). Urinary iPF(2 alpha)-III levels did not differ between day 1 to 5, and were not correlated to cold ischaemia time. Conclusion: Our study shows no evidence of enhanced lipid peroxidation in t he first 5 d following kidney transplantation.