Localization of human interleukin 13 receptor in non-haematopoietic cells

Citation
M. Akaiwa et al., Localization of human interleukin 13 receptor in non-haematopoietic cells, CYTOKINE, 13(2), 2001, pp. 75-84
Citations number
49
Categorie Soggetti
Cell & Developmental Biology
Journal title
CYTOKINE
ISSN journal
10434666 → ACNP
Volume
13
Issue
2
Year of publication
2001
Pages
75 - 84
Database
ISI
SICI code
1043-4666(20010121)13:2<75:LOHI1R>2.0.ZU;2-T
Abstract
Although the functional roles of interleukin (IL-)13 in haematopoietic cell s are well investigated, those in non-haematopoietic cells remain to be add ressed. IL-13 exerts its actions by binding to the IL-13 receptor (IL-13R) on target cells, which is composed of IL-13R alpha1 and the IL-4 receptor a lpha chain (IL-4R alpha), However, there has been no study of localization of IL-13R in each tissue. To address this question, we generated monoclonal anti-IL-13R alpha1 antibody, and performed immunohistochemistry using this antibody and anti-IL-4R alpha antibody. Distribution of these two componen ts was the same in all examined tissues. Staining was positive in keratinoc ytes, hair follicles, and sebaceous and sweat glands in skin; in ciliated r espiratory epithelial cells in nasal tissue; in heart muscle cells; in fove ola cells, gastric glands, and the smooth muscle layer in stomach; and in h epatocytes in liver. However, staining was undetectable in brain and bone m arrow. Fibroblasts and endothelial cells were stained in some tissues. Thes e results provide clues to elucidate the known pathological roles of IL-13 in atopic dermatitis and allergic rhinitis, as well as its unknown physiolo gical roles. (C) 2001 Academic Press.