THE EFFECT OF METHYL LINOLEATE HYDROPEROXIDES AND RADICAL INITIATOR 2,2'-AZOBIS (2-AMIDINOPROPANE) DIHYDROCHLORIDE ON ANTIBODY-PRODUCTION BY MOUSE SPLEEN-CELLS

Methyl linoleate hydroperoxides (MLHPO) and 2,2'-azobis(2-amidinopropa ne) dihydrochloride (AAPH), a widely used free radical initiator, were examined for effects on antibody production by spleen cells using pla que-forming cell response against sheep red blood cells (SRBC). The in vitro primary antibody response was enhanced in the presence of MLHPO at a concentration range of 2-20 mu M or AAPH at 0.1 mu M, but was su ppressed with higher concentrations of these compounds. In the in vitr o secondary antibody response, both MLHPO and AAPH failed to increase plaque-forming cell response above that of the control culture. Follow ing the oral administration of MLHPO (2.29 g/kg) four times to mice, i n vivo primary plaque-forming cell response was significantly suppress ed. After, a single intraperitoneal injection of AAPH (60 mg/kg) to mi ce, in vivo primary plaque-forming cell response was also suppressed. These findings suggest that primary antibody response can be affected by lipid hydroperoxides and oxygen radicals in vivo.