SORBITOL ACCUMULATION AND TRANSMEMBRANE EFFLUX IN OSMOTICALLY STRESSED JS1 SCHWANNOMA CELLS

Authors
MIZISIN AP LI L CALCUTT NA
Citation
Ap. Mizisin et al., SORBITOL ACCUMULATION AND TRANSMEMBRANE EFFLUX IN OSMOTICALLY STRESSED JS1 SCHWANNOMA CELLS, Neuroscience letters, 229(1), 1997, pp. 53-56
Citations number
23
Categorie Soggetti
Neurosciences
Journal title
Neuroscience letters → ACNP
ISSN journal
03043940
Volume
229
Issue
1
Year of publication
1997
Pages
53 - 56
Database
ISI
SICI code
0304-3940(1997)229:1<53:SAATEI>2.0.ZU;2-6
Abstract
Sorbitol accumulation and transmembrane efflux in JS1 schwannoma cells were examined during osmotic stress in the presence of a sorbitol deh ydrogenase (SD) inhibitor and following return to iso-osmotic conditio ns. SD inhibition promoted sorbitol accumulation under hyperglycemic a nd/or hyperosmotic conditions, and sorbitol efflux during iso-osmotic incubation was prevented by cooling to 4 degrees C or by quinidine. It appears that sorbitol levels in JS1 cells are dependent on SD activit y and that sorbitol is rapidly removed upon restoring isoosmotic condi tions. (C) 1997 Elsevier Science Ireland Ltd.