This paper presents a mechanistic, compartmental simulation model of subsur
face-flow constructed wetlands. The model consists of six submodels, includ
ing the nitrogen and carbon cycles, both autotrophic and heterotrophic bact
eria growth and metabolism, and water and oxygen balances. Data from an exi
sting constructed wetland in Maryland were used to calibrate the model. Mod
el results reproduced seasonal trends well. Interactions between the carbon
, nitrogen, and oxygen cycles were evident in model output. In general, eff
luent biochemical oxygen demand, organic nitrogen, ammonium and nitrate con
centrations were predicted well. Because little is known about rootzone aer
ation by wetland plants, oxygen predictions were fair. The model is general
ly insensitive to changes in individual parameters. This is due to the comp
lexity of the ecosystem and the model, as well as the numerous feedback mec
hanisms. The model is most sensitive to changes in parameters that affect m
icrobial growth and substrate use directly. This dynamic, compartmental, si
mulation model is an effective tool for evaluating the performance of subsu
rface-flow constructed wetlands. The model provided insights into treatment
problems at an existing constructed wetland. With further evaluation and r
efinement, the model will be a useful design tool for subsurface-flow const
ructed wetlands. (C) 2001 Elsevier Science B.V. All rights reserved.