Bilayer lipid membrane glucose biosensors with improved stability and sensitivity

Bilayer lipid membranes (BLMs) can be successfully formed on polymers elect rodeposited on a solid metallic support. Avidin-biotin interactions were em ployed for immobilisation of the enzyme on the surface of the BLM. Improved long-term stability and better selectivity towards certain interfering ele ctroactive species were observed for a BLM glucose biosensor based on gluco se oxidase immobilised on a platinum support modified with any of several p olymers. However, the best results were obtained for the mediated system in which the BLM was formed on a Pt support covered with a layer of evaporate d Nafion with incorporated ferrocene. The stable and sensitive response wit h significant elimination of the influence of electroactive interferences o n the signal magnitude should allow a practical application of such miniatu rised biosensors. (C) 2001 Elsevier Science Ltd. All rights reserved.