Pancreatic fate of 6-deoxy-6-[I-125]iodo-D-glucose - In vitro experiments (vol 13, pg 89, 2000)

The apparent distribution space of 6-deoxy-6-[I-125]iodo-D-glucose, recentl y proposed as a tracer of D-glucose transport, was measured in rat isolated islets, acinar tissue, and pieces of pancreas. While such a space reached a steady-state value corresponding to the (HOH)-H-3 volume in pancreatic is lets within 5 min, it slowly increased in pieces of pancreas and, even afte r 60-min incubation, remained lower than the (HOH)-H-3 volume. Moreover, th e net uptake of 6-deoxy-6-[I-125]iodo-D-glucose by pancreatic pieces was in hibited by unlabeled 6-deoxy-6-iodo-D-glucose, D-glucose, and cytochalasin B, while being less or not affected by these agents in isolated islets, A p referential labeling of the endocrine, relative to exocrine, moiety of the pancreas was documented both by comparing, after 2 min incubation, the upta ke of 6-deoxy-6-[I-125]iodo-D-glucose by pieces of pancreas from normal vs streptozotocin-injected rats and by comparing the radioactive content of pa ncreatic islets and acinar tissue obtained from normal rats injected intrav enously 3 min before sacrifice with 6-deoxy-6-[I-125]iodo-D-glucose. It is proposed, therefore, that advantage could conceivably be taken from the vas tly different time course for the uptake of selected monosaccharides by pan creatic islets vs acinar cells in the perspective of imaging of the endocri ne pancreas by a non invasive method.