Identification of two proteins, S14 and UIP1, that interact with UCH37

By the use of the yeast two-hybrid screen we have identified two proteins t hat interacted with UCH37: S14, which is a subunit of PA700 and a novel pro tein, UIP1 (UCH37 interacting protein I). The interaction of UCH37 with S14 or UIP1 was confirmed by in vitro binding assay and in vivo coimmunoprecip itation analysis. The C-terminal extension of UCH37 is essential for intera ction with S14 or UIP1 as shown by the yeast two-hybrid assay and the in vi tro binding assay. Furthermore, UIP1 blocked the interaction between UCH37 and S14 in vitro. (C) 2001 Federation of European Biochemical Societies. Pu blished by Elsevier Science B.V. All rights reserved.