Slow vacuolar channels from barley mesophyll cells are regulated by 14-3-3proteins

The conductance of the vacuolar membrane at elevated cytosolic Ca2+ levels is dominated by the slow activating cation selective (SV) channel. At physi ological, submicromolar Ca2+ concentrations the SV currents are very small. Only recently has the role of 14-3-3 proteins in the regulation of voltage -gated and Ca2+-activated plasma membrane ion channels been investigated in Drosophila, Xenopus and plants. Here we report the first evidence that pla nt 14-3-3 proteins are Involved in the down-regulation of ion channels in t he vacuolar membrane as well. Using the patch-clamp technique we have demon strated that 14-3-3 protein drastically reduces the current carried by SV c hannels, The current decline amounted to 80% and half-maximal reduction was reached within 5 s after 14-3-3-addition to the bath, The voltage sensitiv ity of the channel was not affected by 14-3-3. A coordinating role for 14-3 -3 proteins in the regulation of plasma membrane and tonoplast ion transpor ters is discussed. (C) 2001 Federation of European Biochemical Societies. P ublished by Elsevier Science B.V. All rights reserved.