Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in Fusarium venenatum

Fusarium venenatum JeRS 325, a strain which produces recombinant glucoamyla se under control of a growth rate independent promoter was transformed with a plasmid carrying the Aspergillus niger glucoamylase gene under control o f its own growth rate correlated promoter. Some disruption of the original recombinant genes occurred and at pH 5.8 the double transformant did not pr oduce as much glucoamylase as JeRS 325 in batch culture. However, the doubl e transformant still produced as much glucoamylase as JeRS 325 in fed-batch cultures. illustrating the potential for the combined use of growth rate i ndependent and dependent promoters to improve production of recombinant pro teins in fed-batch culture sq stems. (C) 2001 Federation of European Microb iological Societies. Published by Elsevier Science B.V. All rights reserved .