Novel method for the proteomic investigation of a dairy-associated Bacillus cereus biofilm

The biofilm proteome of a dairy-associated Bacillus cereus strain (B. cereu s 5) was investigated. Biofilm biomass of sufficient concentration for 2D-P AGE was obtained by growing the culture in the presence of glass wool. B. c ereus 5 readily attached to the glass wool and biofilms formed within 18 h. The biofilm proteome of whole-cell proteins revealed that 10 proteins were synthesized as a result of surface attachment of which four were unique to the biofilm profile. Seven proteins appeared to be absent in the biofilm p rofile. The altered proteomes indicated that changes took place in the regu lation of protein expression when B. cereus 5 cells attached to surfaces. ( C) 2001 Federation of European Microbiological Societies. Published by Else vier Science B.V. All rights reserved.