Occurrence of a new metallo-beta-lactamase IMP-4 carried on a conjugative plasmid in Citrobacter youngae from the People's Republic of China

During the course of an antimicrobial resistance surveillance programme in Guangzhou, the People's Republic of China, single strains of Citrobacter yo ungae and Pseudomonas aeruginosa were identified which were resistant to im ipenem and found to carry the carbapenemase gene bla(IMP). PCR screening of the citrobacter strain with specific primers for the bla(IMP) type genes g ave a 587-bp product which when sequenced gave 100% homology with the bla(I MP-4) sequence reported recently from Acinetobacter spp. The determinant in the C, youngae strain was found to be located on a 156-kb plasmid capable of transfer to Escherichia coli UB1637 by conjugation. Sequencing of the bl a(IMP-4) Open reading frame in the C. youngae strain and adjacent sequences not only confirmed the presence of bla(IMP-4) but also identified that a c onserved core site found within the 59-bp element of integrons was present and the same as the one described in the only other occurrence of bla(IMP-4 ) in Acinetobacter spp. isolated from an intensive care unit in Hong Kong. This is the second report of transferable carbapenemase genes in Enterobact er outside of Japan and the first in the people's Republic of China. Under the selective pressure of carbapenems and extended spectrum cephalosporins use we might expect this gene to spread and widespread surveillance should be instituted. (C) 2001 Federation of European Microbiological Societies. P ublished by Elsevier Science B.V. All rights reserved.