Uh. Manjunatha et al., A Mycobacterium smegmatis gyrase B specific monoclonal antibody reveals association of gyrase A and B subunits in the cell, FEMS MICROB, 194(1), 2001, pp. 87-92
DNA gyrase is a unique topoisomerase, which plays important roles in macrom
olecular events like DNA replication, transcription and genetic recombinati
on. In this study a high affinity monoclonal antibody to the gyrase B (GyrB
) subunit of Mycobacterium smegmatis was characterized, which did not cross
-react with either the Escherichia coli GyrB subunit or with GyrB subunits
from other mycobacterial species. The antibody recognized an epitope in the
N-terminus, novobiocin-binding domain of GyrB. Immunoprecipitation of gyra
se from M. smegmatis cell lysate revealed an association, mediated by ionic
interactions, of gyrase A and GyrB subunits in the cell. This antibody is
a valuable tool for structure-function analysis and immunocytological studi
es of myobacterial DNA gyrase. (C) 2001 Federation of European Microbiologi
cal Societies. Published by Elsevier Science B.V. All rights reserved.