Formation, inhibition and clearance of plasmin in vivo

A 5 mug/kg bolus of tissue plasminogen activator (t-PA) was infused into 11 healthy subjects followed by measurement of t-PA activity and antigen, PAI -1 activity and antigen, t-PA/PAI-1 complex, plasmin/antiplasmin (PAP) comp lex and D-dimer over 4 h. Infusion of t-PA resulted in a rise in PAP levels in all subjects from a baseline of 2.4 +/- 1.1 nmol/l to a peak of 5.1 +/- 2.3 nmol/l, but had no effect on plasminogen, antiplasmin or D-dimer level s. Using a kinetic model of plasminogen activation in vivo, the second-orde r rate constant for t-PA binding to plasminogen was estimated to be 3,100 /- 1,300 mol(-1)l.s(-1), 200-500 times slower than t-PA accelerated by fibr in. The half-life of PAP was 4.5 +/- 1.6 h. In healthy subjects, the PAP le vel in plasma represents the average rate of plasminogen activation over th e last 2-8 h. Copyright (C) 2001 S. Karger AG, Basel.