MUTAGENIC RESPONSE TO BENZENE AND TRIS(2,3-DIBROMOPROPYL)-PHOSPHATE IN THE LAMBDA-LACL TRANSGENIC MOUSE MUTATION ASSAY - A STANDARDIZED APPROACH TO IN-VIVO MUTATION ANALYSIS

The genotoxic response of benzene and tris(2,3-dibromopropyl)-phosphat e (TDBP) have been evaluated in several tissues using the standardized lambda/lacl (Big Blue(R)) transgenic mouse mutation assay. Separate g roups of four to five male B6C3F(1) transgenic lambda/lacl mice were g iven oral administrations of benzene or TDBP at varying concentrations . Tissues evaluated include lung, bone marrow, and spleen in benzene-t reated animals, and liver, kidney, and stomach in TDBP-ireaied animals . Significant increases in lacl mutations were observed in the spleen and bone marrow of benzene treated mice, and the kidneys of TDBP-treat ed mice. Where applicable, mutagenesis patterns of tissue sensitivity were consistent with what has been observed previously in other assays . In addition, mutagenicity in tissues not traditionally evaluated For mutations correlated to sites of carcinogenicity for the chemicals te sted. (C) 1996 Wiley-Liss, Inc.