AN EFFICIENT LABORATORY PROTOCOL FOR THE SEQUENCING OF LARGE NUMBERS OF LACL MUTANTS RECOVERED FROM BIG BLUE(R) TRANSGENIC ANIMALS

Mutational spectra provide a powerful approach to investigate both the mutagenic potential and the mechanism of action of suspected mutagens and carcinogens. Recently, transgenic techniques have made it possibl e to generate mutational spectra in animals. Such a spectrum may consi st of 50 to 200 mutants depending on the nature of the mutations, and many spectra can be generated depending on the design of the experimen t. This report describes a practical approach for the processing and s equencing of large numbers of loci mutants recovered from Big Blue(R) animals. (C) 1996 Wiley-Liss, Inc.