DNA shuffling: Modifying the hand that nature dealt

Authors
Citation
Aj. Reid, DNA shuffling: Modifying the hand that nature dealt, IN VITRO-PL, 36(5), 2000, pp. 331-337
Citations number
37
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN journal
10545476 → ACNP
Volume
36
Issue
5
Year of publication
2000
Pages
331 - 337
Database
ISI
SICI code
1054-5476(200009/10)36:5<331:DSMTHT>2.0.ZU;2-F
Abstract
DNA shuffling is a technique being utilized for in vitro recombination of a single gene or pools of homologous genes. The genes are fragmented into ra ndomly sized pieces, and polymerase chain reaction (PCR) reassembly of full -length genes front the fragments, via self-priming, yields recombination d ue to PCR template switching. After these PCR products are screened and the interesting products sequenced, improved clones are reshuffled to recombin e useful mutations in additive or synergistic ways, in effect mimicking the process of natural sexual recombination. Protein can be 'bred' with the ap propriate individual properties and then their 'progeny' screened for the d esired combination of traits. DNA shuffling is a powerful tool enabling rap id and directed evolution of new genes, operons and whole viral genomes.