Mp. Cleary et al., Restoration of fertility in young obese (Lep(ob) Lep(ob)) male mice with low dose recombinant mouse leptin treatment, INT J OBES, 25(1), 2001, pp. 95-97
OBJECTIVE: We investigated the effects of low-dose leptin treatment on rest
oration of fertility in young adult male leptin deficient obese mice.
EXPERIMENTAL DESIGN AND RESULTS: MMTV-TCF-alpha Lep(ob) Lep(ob) mice (8-10
weeks old) were treated with recombinant mouse leptin. In experiment 1, fou
r mice (5 mug/g body weight leptin followed by 2.5 mug/g) lost weight and i
mpregnated females (number of pregnancies/number of females, 3/6, 5/6, 5/10
, 4/10). In experiment 2, Leptin-Obese (2.5 mug/g) and Control-Lean mice we
ighed significantly less than Control-Obese mice. Epididymal pad weights of
Control-Obese mice were the heaviest, followed by those of Leptin-Obese mi
ce, and Control-Lean mice were the lightest. Testes weight was greater in C
ontrol-Lean vs Control-Obese mice. Leptin-Obese mice had testes weight not
significantly different from either control group. Four of five Leptin-Obes
e mice impregnated females (4/10, 5/10, 2/10, 5/12, 0/10).
CONCLUSIONS: These results indicate that low-dosage mouse recombinant lepti
n treatment restored fertility to young Lepob Lep(ob) male mice. Although b
ody weights of Leptin-Obese mice were similar to those of lean age-matched
mice, epididymal fat pad weights were heavier.