Multiple regulatory mechanisms act on the 5 ' untranslated region of the S-layer gene from Thermus thermophilus HB8

Authors
Castan, P de Pedro, MA Risco, C Valles, C Fernandez, LA Schwarz, H Berenguer, J
Citation
P. Castan et al., Multiple regulatory mechanisms act on the 5 ' untranslated region of the S-layer gene from Thermus thermophilus HB8, J BACT, 183(4), 2001, pp. 1491-1494
Citations number
13
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF BACTERIOLOGY
ISSN journal
00219193 → ACNP
Volume
183
Issue
4
Year of publication
2001
Pages
1491 - 1494
Database
ISI
SICI code
0021-9193(200102)183:4<1491:MRMAOT>2.0.ZU;2-O
Abstract
The role of the 5' untranslated region (5'UTR) of the S-layer gene from The rmus thermophilus was analyzed through the isolation of Delta5'UTR mutants. In these mutants the half-life of splA mRNA was strongly reduced and slpA transcription was no longer subjected to growth phase dependent repression. Overproduction and detachment of the external envelopes of the mutants wer e observed in stationary phase.