Control method for integrity of continuous beds

Citation
R. Hahn et A. Jungbauer, Control method for integrity of continuous beds, J CHROMAT A, 908(1-2), 2001, pp. 179-184
Citations number
25
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
Volume
908
Issue
1-2
Year of publication
2001
Pages
179 - 184
Database
ISI
SICI code
Abstract
Monoliths are considered to be the latest version of stationary phases for protein chromatography. They are now becoming popular since they exhibit re duced peak broadening due to low mass transfer resistances. A weak point of monoliths may be an inhomogeneous bed, especially when charged groups are present during co-polymerization. This becomes apparent when a protein solu tion is loaded onto such a monolith. A biphasic breakthrough curve differin g from the sigmoidal-shaped one, is observed. Two error functions were used to approximate the breakthrough curve. From these parameters an estimate o f the fraction of flow passing through the homogeneously packed part of the bed as well as the inhomogeneously one was derived. This flow pattern was also confirmed by perfusing ferritin through an inhomogeneous monolith and comparing it to an intact one. Electron microscopy and mercury porosimetry did not indicate any abnormalities in the bed. (C) 2001 Elsevier Science B. V. All rights reserved.