Kr. O'Connor et Kw. Beyenbach, Chloride channels in apical membrane patches of stellate cells of Malpighian tubules of Aedes aegypti, J EXP BIOL, 204(2), 2001, pp. 367-378
Stellate cells of Aedes aegypti Malpighian tubules were investigated using
patch-clamp methods to probe the route of transepithelial Cl- secretion. Tw
o types of Cl- channel were identified in excised, inside-out apical membra
ne patches. The first Cl- channel, type I, had a conductance of 24 pS, an o
pen probability of 0.816+/-0.067, an open time of 867+/-114 ms (mean +/- S.
E.M., four patches) and the selectivity sequence I->Cl(-)much greater than
isethionate>gluconate. The I-/Cl- permeability ratio was 1.48, correspondin
g to Eisenman sequence I. The type I Cl- channel was blocked by 2,2'-iminod
ibenzoic acid (DPC) and niflumic acid {2-[3-(trifluoromethyl)anilo]nicotini
c acid}. The removal of Ca2+ from the Ringer's solution on the cytoplasmic
side had no effect on channel activity. The second Cl- channel, type II, ha
d a conductance of 8 pS, an open probability of 0.066+/-0.021 and an open t
ime of 7.53+/-1.46 ms (mean +/- S.E.M., four patches). The high density and
halide selectivity sequence of the type I Cl- channel is consistent with a
role in transepithelial Cl- secretion under control conditions, but it rem
ains to be determined whether these Cl- channels also mediate transepitheli
al Cl- secretion under diuretic conditions in the presence of leucokinin.