Multiple outbreaks of Norwalk-like virus gastro-enteritis associated with a Mediterranean-style restaurant

The role of diverse infectious agents, particularly Norwalk-like viruses (N LV), in three successive gastro-enteritis outbreaks in one setting (a resta urant) was evaluated. Methods included standard bacteriological tests, spec ific tests for Escherichia coli, tests for verocytotoxins, electron microsc opy (EM) for viruses and reverse transcription-PCR (RT-PCR) methodology for NLV, No pathogenic bacteria were detected. Verocytotoxin genes, although d etected by PCR in the first outbreak, could not be confirmed in the E, coli isolated, so they did not appear to be of significance, NLV was the main a gent detected in each of the three outbreaks. DNA sequencing and phylogenet ic analysis of the amplified products obtained from the RT-PCR positive spe cimens indicated that only one NLV strain was involved in each outbreak, bu t the NLV strains responsible for the three outbreaks were different from e ach other. PCR technology for detection of NLV proved highly sensitive, but failed to detect one specimen which was positive by EM, The restaurant ass ociated with the outbreaks is a Mediterranean-style restaurant where food f rom a common platter is typically eaten with fingers. The findings indicate that NLV was introduced by guests or staff and was not due to a long-term reservoir within the setting.