Detection of secondary genetic aberrations in follicle center cell derivedlymphomas: assessment of the reliability of comparative genomic hybridization and standard chromosome analysis
A. Viardot et al., Detection of secondary genetic aberrations in follicle center cell derivedlymphomas: assessment of the reliability of comparative genomic hybridization and standard chromosome analysis, LEUKEMIA, 15(1), 2001, pp. 177-183
Secondary chromosomal aberrations in follicle center cell derived lymphomas
(FCDL) usually involve gains and losses of genetic material and may be an
important prognostic value. In the present study, we aimed to determine the
power of comparative genomic hybridization (CGH) as compared to standard c
hromosome analysis (CA) to detect such secondary aberrations. The same lymp
h node cell suspensions prepared from 30 patients with FCDL were analyzed i
n parallel by CGH and CA based on R banding. In all, 73 discrepancies were
found. Sixty-two imbalances were detected only by CA and 11 only by CGH, In
cases with completely resolved karyotypes (n = 17), the median number of d
iscrepancies between CGH and CA was one. However, when the karyotype was pa
rtially resolved (n = 12), the median was four (P < 0.01), Discrepant resul
ts were further studied by fluorescence in situ hybridization using locus-s
pecific probes. These data confirm, that not only for the detection of bala
nced aberrations, but also for the detection of unbalanced aberrations in F
CDL, standard chromosome analysis is still the 'gold standard'. In contrast
, CGH is useful to detect chromosomal imbalances when no metaphases are fou
nd or no fresh material is available.