Molecular cloning and characterization of a novel phospholemman-like protein from rat hippocampus

A cDNA encoding a putative ion channel protein was isolated from a rat hipp ocampus library. This gene, termed phosphohippolin (Php), contains 318 bp o pen reading frame encoding a single transmembrane protein with a 5' signal peptide region. The deduced amino acid sequence shares 48.1% homology with phospholemman (Plm). Expression sequence tag database (dEST) search identif ied a mouse (AA521976) and human (AA209241) Php gene homologues. The tissue distribution studies of Php mRNA showed its abundant expression in rat bra in and kidney, and in the brain, high expression was observed in hippocampu s and cerebellum. (C) 2001 Elsevier Science B.V. All rights reserved.