Immunological identification of the mammalian H-3 histamine receptor in the mouse brain

Affinity-purified antibodies raised against the peptide sequence H-3 (349-3 58) receptor specifically recognized Mo protein species with M-r 62 000 and 93 000 in adult mouse forebrain membranes. Both immunoreactive species wer e suppressed greatly by preincubation of the antibody with the respective p eptide. Immunohistochemical analysis using affinity-purified anti-H-3 (349- 358) antibodies yielded a high degree of coincidence with ligand-autoradiog raphical information, with high levels detected in the CA3 and dentate gyru s of the hippocampus, laminae V of the cerebral cortex, the olfactory tuber cle. Purkinje cell layer of the cerebellum, substantia nigra, globus pallid us, thalamus and striatum. This study suggests further biochemical evidence for multiple H-3 receptor subtypes and the widespread distribution of-the H-3 receptor in the mammalian brain. NeuroReport 12:259-262 (C) 2001 Lippin cott Williams & Wilkins.