K. Finster et al., DESULFUROMONAS THIOPHILA SP-NOV, A NEW OBLIGATELY SULFUR-REDUCING BACTERIUM FROM ANOXIC FRESH-WATER SEDIMENT, International journal of systematic bacteriology, 47(3), 1997, pp. 754-758
A mesophilic, acetate-oxidizing, sulfur-reducing bacterium, strain NZ2
7(T), was isolated from anoxic mud from a freshwater sulfur spring, Th
e cells were ovoid, motile, and gram negative, In addition to acetate,
the strain oxidized pyruvate, succinate, and fumarate, Sulfur flower
could be replaced by polysulfide as an electron acceptor, Ferric nitri
lotriacetic acid was reduced in the presence of pyruvate; however, thi
s reduction did not sustain growth. These phenotypic characteristics s
uggested that strain NZ27(T) is affiliated with the genus Desulfuromon
as. A phylogenetic analysis based on the results of comparative 16S ri
bosomal DNA sequencing confirmed that strain NZ27(T) belongs to the De
sulfuromonas cluster in the recently proposed family ''Geobacteraceae'
' in the delta subgroup of the Proteobacteria, In addition, the result
s of DNA-DNA hybridization studies confirmed that strain NZ27(T) repre
sents a novel species, Desulfuromonas thiophila, a name tentatively us
ed in previous publications, is the name proposed for strain NZ27(T) i
n this paper.