REARRANGEMENTS IN THE GENOMES OF VIBRIO-CHOLERAE STRAINS BELONGING TODIFFERENT SEROVARS AND BIOVARS

Citation
S. Nandi et al., REARRANGEMENTS IN THE GENOMES OF VIBRIO-CHOLERAE STRAINS BELONGING TODIFFERENT SEROVARS AND BIOVARS, International journal of systematic bacteriology, 47(3), 1997, pp. 858-862
Citations number
19
Categorie Soggetti
Microbiology
ISSN journal
00207713
Volume
47
Issue
3
Year of publication
1997
Pages
858 - 862
Database
ISI
SICI code
0020-7713(1997)47:3<858:RITGOV>2.0.ZU;2-D
Abstract
The intron-encoded enzyme I-CeuI provides an excellent tool for rapidl y examining the organization of genomes of related species of bacteria . Vibro cholerae strains belonging to serovars O1 and O139 have 9 I-Ce uI sites in their genomes, and V. cholerae strains belonging to serova rs non-O1 and non-O139 have 10 I-CeuI sites in their genomes, This inf ormation can be used as a criterion to differentiate O1 strains from n on-O1 and non-O139 strains, To our knowledge, intraspecies variation i n the number of rrn operons has not been reported in any other organis m, Our data revealed extensive restriction fragment length polymorphis m based on a comparison of the I-CeuI digestion profiles of strains be longing to different serovars and biovars, From the analysis of partia l digestion products, I-CeuI macrorestriction maps of several classica l, El Tor, and O139 strains were constructed. While the linkage maps a re conserved within biovars, linkage maps vary substantially between b iovars.