DETERMINATION OF CEFUROXIM LEVELS IN HUMAN SERUM BY MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY WITH DIRECT SAMPLE INJECTION

Effective monitoring of cefuroxim levels by micellar electrokinetic ca pillary chromatography with direct serum injection are discussed and c ompared with the HPLC method. With capilliary electrophoresis (CE), in contrast to HPLC, good resolution and efficiency was demonstrated as well as low consumption of solvent and samples. The CE system was appl ied at 15 kV with UV detection at 274 nm using 150 mM sodium dodecyl s ulfate in 20 mM sodium phosphate and berate (pH 9.0) as electrolyte. T he results were seen within 12 min with efficiency approaching 182 000 theoretical plates. The coefficients of variations of migration time and peak area were less than 0.8 and 5.9%, respectively. The detection limits for quantitative determination were 0.28 mu M level. Good line arity and recovery were also obtained in the range of serum levels usu ally encountered in clinical analysis with a correlation coefficient o f r = 0.991 and 98-101% recovery. The monitoring of cefuroxim in human serum with micellar electrokinetic capilliary chromatography (MECC) i s demonstrated. Identification of cefuroxim in human serum with MECC i s demonstrated. Identification of cefuroxim was performed by character izing the sample peak in terms of the migration time and UV spectrum. Considering the results of our study, the CE method should be highly s uitable for the separation of cefuroxim in biofluids. (C) 1997 Elsevie r Science B.V.