CHROMATOGRAPHY OF CROTAMITON AND ITS APPLICATION TO THE DETERMINATIONOF ACTIVE INGREDIENTS IN OINTMENTS

Crotamiton, which is a mixture of cis and trans isomers, was investiga ted by several separation techniques. One of the HPLC modes, in which crotamiton eluted as a single peak, was selected for the determination of five active ingredients (crotamiton, prednisolone, glycyrrhetinic acid, dibucaine and chlorhexidine hydrochloride) in an ointment. The s imultaneous determination was performed using isocratic reversed-phase mode within 20 min by employing an octyl (C-8) column and a mobile ph ase containing sodium dodecyl sulfate (SDS) and 2-propanol. The method was successfully applied to quality control and stability testing of the ointment. (C) 1997 Elsevier Science B.V.