ANALYSIS OF IBUPROFEN METABOLITES BY SEMI-MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET-ABSORPTION AND PULSED AMPEROMETRIC DETECTORS

Authors
KIMURA T SHIROTA O OHTSU Y
Citation
T. Kimura et al., ANALYSIS OF IBUPROFEN METABOLITES BY SEMI-MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET-ABSORPTION AND PULSED AMPEROMETRIC DETECTORS, Journal of pharmaceutical and biomedical analysis, 15(9-10), 1997, pp. 1521-1526
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
15
Issue
9-10
Year of publication
1997
Pages
1521 - 1526
Database
ISI
SICI code
0731-7085(1997)15:9-10<1521:AOIMBS>2.0.ZU;2-7
Abstract
Semi-microcolumn (1.5 mm i.d.) liquid chromatography (LC) system with ultraviolet (UV) and pulsed amperometric detector (PAD) was constructe d for analysis of ibuprofen metabolites in human urine. PAD was connec ted in series with the UV detector, and an alkaline solution was post- column added to a mobile phase after the UV detector. By a gradient el ution, five ibuprofen metabolites were detected with UV detection from 1 mu l of human urine at 3.5 h after the administration, and informat ion concerning their glucuronation was simultaneously obtained by PAD response. (C) 1997 Elsevier Science B.V.