T. Kimura et al., ANALYSIS OF IBUPROFEN METABOLITES BY SEMI-MICROCOLUMN LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET-ABSORPTION AND PULSED AMPEROMETRIC DETECTORS, Journal of pharmaceutical and biomedical analysis, 15(9-10), 1997, pp. 1521-1526
Semi-microcolumn (1.5 mm i.d.) liquid chromatography (LC) system with
ultraviolet (UV) and pulsed amperometric detector (PAD) was constructe
d for analysis of ibuprofen metabolites in human urine. PAD was connec
ted in series with the UV detector, and an alkaline solution was post-
column added to a mobile phase after the UV detector. By a gradient el
ution, five ibuprofen metabolites were detected with UV detection from
1 mu l of human urine at 3.5 h after the administration, and informat
ion concerning their glucuronation was simultaneously obtained by PAD
response. (C) 1997 Elsevier Science B.V.