Plant regeneration of Fraxinus angustifolia by in vitro shoot organogenesis

Citation
G. Tonon et al., Plant regeneration of Fraxinus angustifolia by in vitro shoot organogenesis, SCI HORT A, 87(4), 2001, pp. 291-301
Citations number
25
Categorie Soggetti
Plant Sciences
Journal title
SCIENTIA HORTICULTURAE
ISSN journal
03044238 → ACNP
Volume
87
Issue
4
Year of publication
2001
Pages
291 - 301
Database
ISI
SICI code
0304-4238(20010312)87:4<291:PROFAB>2.0.ZU;2-5
Abstract
The in vitro regeneration of Fraxinus angustifolia through bud/shoot organo genesis on embryonic explants was investigated. Embryo axes and cotyledons from mature seeds underwent adventitious regeneration after exposure to gro wth regulators, the embryo axes showing a greater regenerative potential th an cotyledons. While the MS medium proved effective for the induction and i nitiation phases, it was inadequate for bud development, and was replaced b y DKW medium. A constant supply of benzyladenine (BA) appeared essential fo r all phases. The gelling agents used had an important role in morphogenesi s: Duchefa Gelrite positively affected the number of new buds per explant. The rooting of developed shoots was easily achieved on auxin-free WPM mediu m and most of the rooted plantlets "hardened" after a period of acclimation in a mist greenhouse. (C) 2001 Elsevier Science B.V. All rights reserved.