Variation in liposome binding among enteroviruses

Liposome-binding properties of native virions and in vitro generated 135S p articles of eight enteroviruses were studied. The temperature required for the structural transition from the native 160S virion to a 135S particle wa s virus-specific, ranging from +38 degreesC to more than +50 degreesC. Whil e the 135S particles of poliovirus 1/Mahoney (PV1) and coxsackievirus A21 ( CAV21) were capable of binding to liposomes, the other viruses showed minim al binding. Both of the viruses that bound to liposomes as 135S particles a lso bound as native virions. In addition, PV3/Sabin bound to liposomes as n ative virions but not as 135S particles, and the flotation patterns were di fferent from those of PV1 and CAV21. The nonbinding viruses included coxsac kieviruses A7, A9, A16, and B5, and enterovirus 68. The results follow the new classification of enteroviruses, as CAV21 is a member of the human ente rovirus C species, which is genetically close to the poliovirus species. (C ) 2001 Academic Press.