MULTIPLE IMMUNOBLOTS AFTER NON-ELECTROPHORETIC BIDIRECTIONAL TRANSFEROF A SINGLE SDS-PAGE GEL WITH MULTIPLE ANTIGENS

Western blotting is a very sensitive and powerful fundamental techniqu e in immunology that has been used to detect and characterize proteins of low abundance. This technique employs the transfer of proteins sep arated on SDS-PAGE to nitrocellulose sheets for further detection usin g antibodies. Here we report the non-electrophoretic transfer of the 6 0-kDa Ro (or SSA) autoantigen, 240 and 220 kDa spectrin antigens and p restained molecular weight standards from SDS-PAGE gels to nitrocellul ose to obtain multiple immunoblots. In fact, we have used this procedu re to obtain 12 immunoblots from a single gel with multiple sera.