Mapping of 14 expressed sequence tags (ESTs) from porcine skeletal muscle by somatic cell hybrid analysis

Chromosomal assignments are reported fur fourteen porcine expressed sequenc e tags (ESTs) - CALM1, CRYAB, MYH7, MYL1, PDK4, PGAM2, PYGM, REV3L, RFC1, S LN, SPTBN1, SRM160, TPM1 and YWHAG. The ESTs were derived from our porcine skeletal muscle cDNA library. The ESTs sequences selected for mapping inclu ded the presence of the 3'-untranslated region. The assignments were perfor med using two independent somatic cell hybrid panels providing the possibil ity of confirmation of the results obtained. The observed localizations are compared with the locations predicted from heterologous (human-pig, pig-hu man) chromosome painting data and knowledge of the map locations of the hum an homologues. These results add new information to the porcine genome tran script map.