Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria

Authors
Taghavi, S Delanghe, H Lodewyckx, C Mergeay, M van der Lelie, D
Citation
S. Taghavi et al., Nickel-resistance-based minitransposons: New tools for genetic manipulation of environmental bacteria, APPL ENVIR, 67(2), 2001, pp. 1015-1019
Citations number
25
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
67
Issue
2
Year of publication
2001
Pages
1015 - 1019
Database
ISI
SICI code
0099-2240(200102)67:2<1015:NMNTFG>2.0.ZU;2-X
Abstract
The ncc and nre nickel resistance determinants from Ralstonia eutropha-like strain 31A were used to construct mini-Tn5 transposons. Broad host express ion of nickel resistance was observed for the nre mini-transposons in membe rs of the alpha, beta, and gamma subclasses of the Proteobacteria, while th e ncc minitransposons expressed nickel resistance only in R. eutropha-like strains.