A polyphasic taxonomic study involving DNA-DNA hybridization, whole-cell pr
otein electrophoresis, and 16S ribosomal DNA sequence analysis revealed tha
t a group of Burkholderia cepacia-like organisms isolated from the rhizosph
ere or tissues of maize, wheat, and lupine belong to B. cepacia genomovar I
II, a genomic species associated with "cepacia syndrome" in cystic fibrosis
patients. The present study also revealed considerable protein electrophor
etic heterogeneity within this species and demonstrated that the B. cepacia
complex consists of two independent phylogenetic lineages.