S. Chang et al., Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation, AQUACULTURE, 192(2-4), 2001, pp. 133-145
A tropical marine fish cell line (SF) was established from Asian seabass (L
ates calcarifer) fry. The cell line was maintained in Earle's minimum essen
tial medium (EMEM) supplemented with foetal calf serum and incubated at 25
degreesC, The susceptibility of the cell-line at the 16th, 35th and 46th su
bculture to several iridoviruses, birnaviruses, reoviruses, a rhabdovirus,
and a nodavirus was studied. Cytopathic effect (CPE) was observed daily aft
er virus inoculation and viral replication efficiency was determined for si
x viruses. SF cell cultures infected with an iridovirus, a nodavirus and a
reovirus were further elucidated by electron microscopy. All the viruses te
sted were shown to induce CPE on SF cells. This suggests that the SF cell l
ine has good potential for the isolation of various fish viruses. The SF ce
ll line consists predominantly of epithelial-like cells and has been subcul
tured 85 times over a period of 24 months. (C) 2001 Elsevier Science B.V. A
ll rights reserved.