Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation

Citation
S. Chang et al., Development of a tropical marine fish cell line from Asian seabass (Lates calcarifer) for virus isolation, AQUACULTURE, 192(2-4), 2001, pp. 133-145
Citations number
23
Categorie Soggetti
Aquatic Sciences
Journal title
AQUACULTURE
ISSN journal
00448486 → ACNP
Volume
192
Issue
2-4
Year of publication
2001
Pages
133 - 145
Database
ISI
SICI code
0044-8486(20010115)192:2-4<133:DOATMF>2.0.ZU;2-R
Abstract
A tropical marine fish cell line (SF) was established from Asian seabass (L ates calcarifer) fry. The cell line was maintained in Earle's minimum essen tial medium (EMEM) supplemented with foetal calf serum and incubated at 25 degreesC, The susceptibility of the cell-line at the 16th, 35th and 46th su bculture to several iridoviruses, birnaviruses, reoviruses, a rhabdovirus, and a nodavirus was studied. Cytopathic effect (CPE) was observed daily aft er virus inoculation and viral replication efficiency was determined for si x viruses. SF cell cultures infected with an iridovirus, a nodavirus and a reovirus were further elucidated by electron microscopy. All the viruses te sted were shown to induce CPE on SF cells. This suggests that the SF cell l ine has good potential for the isolation of various fish viruses. The SF ce ll line consists predominantly of epithelial-like cells and has been subcul tured 85 times over a period of 24 months. (C) 2001 Elsevier Science B.V. A ll rights reserved.