Complete nucleotide sequence of RNA-2 of Olive latent ringspot virus

The complete nucleotide sequence of Olive latent ringspot virus (OLRSV) RNA -2 was determined. This RNA is 3969 nucleotides in length and contains a si ngle open reading frame of 3448 nt, that encodes a polypeptide of 1146 amin o acids, with a calculated M-r of 126,044. OLRSV RNA-2 has a structural org anization typical of nepoviruses, with the coat protein (CP) cistron locate d in the C-terminal and the putative movement protein (MP) in the N-termina l regions of the polyprotein. Computer-assisted comparison of coat proteins of OLRSV and other nepoviruses disclosed relationships that tally with sub grouping based on physicochemical properties.