Complete sequences and phylogenetic analyses of a Singapore isolate of broad bean wilt fabavirus

The complete nucleotide (nt) sequence of a Singapore isolate of broad bean wilt fabavirus from Megakepasma erythrochlamys L., designated BBWVME, was d etermined. Its bipartite genome consisted of two positive-sense singlestran ded ribonucleic acids (RNA). RNA1 (5951 nt in length) encoded a putative pr otease cofactor, nucleotide triphosphate (NTP)-binding domain (helicase), v iral genome-linked protein (VPg), protease and RNA-dependent RNA polymerase (RdRp). RNA2 (3607 nt in length) encoded a putative movement protein (MP) and coat proteins (CP). Genome organization of BBWV-ME was similar to other viruses in the Comoviridae family. Phylogenetic analyses showed that fabav iruses were more closely related to the comoviruses than the nepoviruses.