Amplification and analysis of DNA flanking known sequences of a novel herpesvirus from green turtles with fibropapilloma - Brief report

A 1,632-bp fragment, flanking the original 483-bp region of the DNA polymer ase gene of a novel herpesvirus found in tissues of green turtles (Chelonia mydas) with fibropapilloma, was amplified from the circularized EcoRI-cut DNA extracted from tumor tissues by inverse PCR. The resultant 2,019-bp par tial sequence of the DNA polymerase gene of the newfound herpesvirus, inclu ding the original 483-bp region, showed a high degree of homology at both t he nucleotide and amino acid levels with that of other human and animal her pesviruses. Phylogenetic analysis confirmed that this novel herpesvirus bel onged to the Alphaherpesvirinae subfamily.