CLONING AND CHARACTERIZATION OF THE RAT P130, A MEMBER OF THE RETINOBLASTOMA GENE FAMILY

A cDNA clone encoding rat p130, a member of the retinoblastoma (Rb) ge ne family, was isolated based on the sequence homology of the E1A-bind ing domain. The 4.87 kb cDNA contained an 1135-amino acid open reading frame with high homologies to the human and mouse p130 and a partial homology to the pRb protein. p130 showed difference in distribution of potential phosphorylation sites from pRb in the N-terminal and the B pocket regions. p130 mRNA was detected in most rat tissues. The p130 g ene was mapped to rat chromosome 19p11-13 by fluorescence in situ hybr idization. (C) 1997 Elsevier Science B.V.