Effects of selected herbicides on the germination and infection process ofPuccinia lagenophora, a biocontrol pathogen of Senecio vulgaris

The herbicides 2,PD, glyphosate, linuron, and MCPP at rates of IX (recommen ded held rate), 0.25X, 0.025X, and 0.0125X were evaluated in vitro for thei r effects on the rust fungus Puccinia lagenophorae, a biocontrol agent for the annual weed Senecio vulgaris. Herbicides applied at 1X and 0.25X comple tely prevented aeciospore germination. Glyphosate was toxic even at 0.0125X and 0.025X. Aeciospores germinated in linuron, 2,4-D, and MCPP at 0.025X a nd 0.0125X at rates similar to the water control. Abnormal germ-tube growth was observed with 2,4-D at 0.25X and 0.025X, with linuron at 0.025X, and w ith glyphosate at 0.0125X. Further in planta studies were perfomed with two inbred lines of S. vulgaris inoculated with aeciospores of P. lagenophorae and treated with water, linuron, and 2,4-D at 0.025X at different times of application. Quantitative analysis of the infection process revealed that both herbicides reduced spore deposition on the leaves and altered leaf mor phology. The herbicides had no effect on disease severity at this low rate although linuron significantly reduced the formation of infection peg. Timi ng of herbicide application had no influence on the infection process, and the effect of the herbicides on fungal development did not differ between t he two plant lines. Thus, the herbicides applied at 0.025X did not increase plant susceptibility to the rust fungus, and the rates of 1X, 0.25X, and 0 .025X would prevent, inhibit, or delay fungal development. Therefore, joint application of P. lagenophorae with these herbicides to control S. vulgari s cannot be recommended, (C) 2001 Academic Press.