Study of cell-material interaction by estimating NF-kappa B activation in HeLaS3 cells adhered onto hydrophilic substrates

In order to interpret how cells recognize biomaterials, nucleic factor-kapp a B (NF-kappaB) activation in the attached HeLa S3 cells on various substra tes was evaluated. As substrates, materials of hydrophilic nature (cellulos e, poly(acrylamide)-grafted poly(ethylene) (PAAm-g-PE), and lipids films) w ere used. The contemporary assay method for NF-kappaB was modified to fit o ur system. As a result, NF-kappaB activation varied depending on the substr ates. The NF-kappaB outcome was induced significantly in the HeLa S3 cells that had adhered onto the lipid films in a short time. On the other hand, h igh levels of NF-kappaB induction were observed in the HeLa cells adhered t o the celluose and PAAm-g-PE after a 24 h incubation period. The induction of NF-kappaB by cell-material interaction is discussed from the point of vi ew of biocompatibility. (C) 2001 Elsevier Science Ltd. All rights reserved.