Reduced glutathione, dithiothreitol and cytochrome P-450 inhibitors do notinfluence hypoxic chemosensory responses in the rat carotid body

Citation
A. Roy et al., Reduced glutathione, dithiothreitol and cytochrome P-450 inhibitors do notinfluence hypoxic chemosensory responses in the rat carotid body, BRAIN RES, 889(1-2), 2001, pp. 131-137
Citations number
25
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
00068993 → ACNP
Volume
889
Issue
1-2
Year of publication
2001
Pages
131 - 137
Database
ISI
SICI code
0006-8993(20010119)889:1-2<131:RGDACP>2.0.ZU;2-R
Abstract
Glomus cells and carotid sinus afferents are anatomically connected, and th e chemical events in the glomus cells are expected to be conveyed reflexly as afferent signals. Accordingly, K+ channel inhibition of the glomus cell membrane is expected to be followed by excitation of the afferents. In orde r to test the redox inhibition of K+ channels of glomus cells by reduced gl utathione (GSH), dithiothreitol (DTT) and by cytochrome P-450 inhibitors (c lotrimazole and miconazole), we measured the carotid sinus nerve (CSN) disc harge using an in vitro perfused adult rat carotid body (CB) in the presenc e and absence of these chemicals which are expected to excite the afferents . Our findings were that these agents did not stimulate the CSN activities during normoxia and kept the hypoxic responses intact. These results led us to conclude that the redox modulation of glomus cells was not conveyed to the afferents, and this functional disconnection did not support the redox hypothesis of O-2 chemorecrption in the whole carotid body. (C) 2001 Elsevi er Science B.V. All rights reserved.